Original Articles

Review on the Approaches of Separ ating Autotrophic and Heterotrophic Components of Soil Respir ation

  • 1. Institute of Geographical Science and Natural Resources Research, CAS, Beijing 100101, China|
    2. Graduate School of Chinese Academic Science, Beijing 100039

Received date: 2006-03-01

  Revised date: 2006-06-01

  Online published: 2006-07-25


Soil respiration is an important part of global carbon cycle, which accounts for about 25% of the global carbon dioxide exchange. Estimating the contribution of soil organic material ( SOM- derived ) respiration, root respiration and rhizomicrobial respiration to total soil CO2 efflux and the carbon distribution between actual root respiration and rhizodeposits is crucial in determining the carbon and energy balance of plant and soil. Up to date a large amount of the researches have been done on partitioning total soil respiration into autotrophic and heterotrphic components and great progress has been achieved. But it is still very difficult to discriminate between the CO2 directly derived from actual root respiration and that derived from mineralization of rhizodeposits, and this has presented one of the greatest challenges to quantifying rhizosphere C- flow. Several methods have been suggested to separate SOM- derived respiration and root- derived respiration (including actual root respiration and rhizomicrobial respiration): (1) integration of component method, (2) trenching method, (3) root- excising method, (4) gap formation method, (5) root mass extrapolation method, and (6) isotope method. Also there are several methods to separate actual root respiration and rhizomicrobial respiraton: (1) isotope dilution method, (2) model rhizodeposition method, (3) 14CO2 dynamics method, (4) exudate elution method, (5) 13C of CO2 and microbial biomass and (6) combination of some no- isotopic methods. This review has described the basic principles and assumptions of these methods and compared the results obtained in the original research papers.

Cite this article

JIN Zhao,DONG Yunshe,QI Yuchun . Review on the Approaches of Separ ating Autotrophic and Heterotrophic Components of Soil Respir ation[J]. PROGRESS IN GEOGRAPHY, 2006 , 25(4) : 22 -33 . DOI: 10.11820/dlkxjz.2006.04.003


[1] Schlesinger W H. Biogeochemistry: an analysis of global change. 2nd Edn. Academic Press, San Diego, 1997, 588.

[2] Marland G, Bode T A, Andres R, J Global, regional and national CO2 emission, in: Trends: A Compendium of data on global change. Carbon dioxide information analysis center. Oak Rige National Laboratory, US Department of Energy, Oak Rige, Tennessee, 2001.

[3] Bouwmann A F, Germon J C. Special issue: soils and climate change: introduction. Biology and Fertility of Soils, 1998, 27: 219.

[4] 彭少麟. 全球变化条件下的土壤呼吸效应. 地球科学进展, 2002, 17(5): 705~713.

[5] Schimel D S. Terrestrial ecosystems and the carbon cycle. Global Change Biology , 1995, 1: 77~91.

[6] Schlesinger W H, J A Andrews. Soil respiration and the global carbon cycle. Biogeochemistry, 2000, 48:7~20.

[7] James W R, Christopher S P, Dwipen B. Interannual variability in global soil respiration , 1980~1994. Global Biology Change, 2002, 8: 800~812.

[8] Cao Ming Kui, Tao Bo, Li Ke Rang, Shao Xue Mei, Stephen D P. Interannual Variation in Terrestrial Ecosystem Carbon Fluxes in China from 1981 to 1998, Acta Botanica Sinica 2003,45(5): 552~560.

[9] Kuzyakov Y, H Ehrensberger, K Stahr. Carbon partitioning and below- ground translocation by Lolium perenne. Soil Biology and Biochemistry, 2001, 33 : 61~74.

[10] Kuzyakov Y. Separating microbial respiration of exudates from root respiration in non- sterile soils: a comparison of four methods. Soil Biology and Biochemistry , 2002, 34:1621~1631.

[11] Kuzyakov Y. Theoretical background for partitioning of root and rhizomicrobial respiration by δ13C of microbial biomass. European Journal of Soil Biology, 2005a, doi:10.1016/j.ejsobi.2005.07.002, in press.

[12] Kuzyakov Y, Larionova A A. Root and rhizomicrobial respiration: A review of approaches to estimate respiration by autotrophic and heterotrophic organisms in soil. Journal of Plant Nutrition and Soil Science, 2005b, 168(4):503~520.

[13] Killham K, Yeomans C. Rhizosphere carbon flow measurement and implications: from isotopes to reporter genes. Plant and Soil, 2001,232: 91~96.

[14] Hanson P J, Edwards N T, Garten C T, Andrews J A. Separating root and soil microbial contributions to soil respiration: A review of methods and observations. Biogeochemistry, 2000, 48:115~146.

[15] Dormaar J F. Effect of active roots on the decomposition of soil organic matter. Biology Fertility Soils 1990, 19: 121~126.

[16] Kuzyakov Y. Review: factors affecting rhizosphere priming effects. Plant nutrition and soil science. 2002, 165: 382~396.

[17] Cheng W, Kuzyakov Y. Root effects on decomposition of organic matter. In: Wright, S. (ed.): Roots and Soil management: Interactions Between Roots and Soils. Soil Science Society of America Book Series, Soil Science Society of America, Inc., Madison, Wisconsin, USA, 2005, in press.

[18] Lynch J M, and Whipps J M. Subtrate flow in the rhizosphere. In: Keister D, Cregan P. (eds.): The rhizosphere and plant growth. Kluwer Academic publishers, Dordrecht, 1991, 15~24 .

[19] Kuzyakov Y, Domanski G. Model for rhizodeposition and CO2 efflux from planted soil and its validation by 14C pulse labeling of ryegrass. Plant and Soil, 2002, 239: 87~102.

[20] Nguyen C. Rhizodeposition of organic carbon by plants: mechanisms and controls. Agronomie, 2003, 23: 375~396.

[21] Bazin M J, Markham P, Scott E M, Lynch J M. Population dynamics and rhizosphere interactions. In: Lynch J M. (ed.): The Rhizosphere, John Wily, New York, 1990, 99~127.

[22] Raich J W, Mora M. Estimating root plus rhizosphere contributions to soil respiration on annual croplands. Soil Science and Society of American Journal, 2005, 69: 634~639.

[23] Kelting D L, James A B, Gerry S E. Estimating root respiration, microbial respiration in the rhizosphere, and root- free soil respiration in forest soils. Soil Biology and Biochemistry, 1998, 30(7): 961~968.

[24] 刘立新, 董云社, 齐玉春. 草地生态系统土壤呼吸研究进展. 地理科学进展, 2004, (23)4: 35~42.

[25] 杨玉盛, 董彬, 谢锦升, 陈光水, 李灵, 刘东霞, 李震. 林木根呼吸及测定方法进展. 植物生态学报, 2004, 28(3): 426~434.

[26] 易志刚, 等. 土壤各组分呼吸区分方法研究进展. 生态学杂志, 2003, 22(2):65~69.

[27] 程慎玉, 张宪洲. 土壤呼吸中根系与微生物呼吸的区分方法与应用. 地球科学进展, 2003,18(4): 598~602.

[28] Coleman D C. Compartmental analysis of total soil respiration: an exploratory study. Oikos,1973, 24: 361~366.

[29] Edwards N T, Sollins P. Continuous measurement of carbon dioxide evolution from partitioned forest floor components. Ecology, 1973,54: 406~412.

[30] Powlson D S. The effects of grinding on microbial and non- microbial organic matter in soil. Journal of Soil Science, 1980, 31: 77~85.

[31] Burton A J, Pregitzer K S. Measurement carbon dioxide concentration does not affect root respiration of nine tree species in the field. Tree Physiology, 2002, 22: 67~72.

[32] Epron D, Farque L, Lucot E, Badot P .M. Soil CO2 efflux in a beech forest, the contribution of root respiration. Annual of Forest Science,1999, 56: 289~295.

[33] Buchmann N. Biotic and abiotic factors controlling soil respiration rates in Picea abies stands. Soil Biology and Biochemistry, 2000, 32: 1625~1635.

[34] Lee M S, Nakane K, Nakatsubo T, Koizumi H, Abe J. Seasonal changes in the contribution of root respiration to total soil respiration in a cool- temperate deciduous forest. Plant and Soil, 2003, 255:311~318.

[35] 陈光水, 杨玉盛, 王小国, 谢锦升, 高人, 李震. 格氏栲天然林与人工林根系呼吸季节动态及影响因素. 生态学报, 2005, 25(8): 1941~1947.

[36] 张宪权, 王文杰, 祖元刚, 张万里. 东北地区几种不同林分土壤呼吸组分的差异性. 东北林学大学学报, 2005, 33(2): 46~73.

[37] Hall A J, Connor DJ, Whitfield D M. Root respiration during grain filling in sunflower: the effects of water stress. Plant and Soil, 1990, 121: 57~66.

[38] Edwards N T, Norby R J. Below- ground respiratory responses of sugar maple and red maple saplings to atmospheric CO2 enrichment and elevated air temperature. Plant and Soil, 1998, 206: 85~97.

[39] Rochette P, Flanagan L B, Gregorich E G. Separating soil respiration into plant and soil components using analyses of the natural abundance of carbon_13. Soil Science Society ofAmerica Journal, 1999, 63:1207~1213.

[40] Brumme R. Mechanisms of carbon and nutrient release and retention in beech forest gaps. Plant and Soil, 1995, 168/169: 593~600.

[41] Nakane K, Kohno T, Horikoshi T. Root respiration before and just after clear- felling in a mature deciduous, broad- leaved forest. Ecology Research, 1996, 11: 111~119.

[42] 李凌浩, 韩兴国, 王其兵,等.锡林河流域一个放牧草原群落中根系呼吸占土壤总呼吸比例的初步估计. 植物生态学报, 2002, 26(1): 29~32.

[43] Kucera C, Kirkham D. Soil respiration studies in tall grass prair in Missouri.Ecology,1971,52: 912~915.

[44] Schonwitz R, Stichler W, Ziegler H. 13C values of CO2 from soil respiration on sites with crops of C3 and C4 type photosynthesis. Oecologia ,1986, 69: 305~308.

[45] Coleman D C, Fry B.
[eds]: Carbon Isotope. Techniques, Academic Press, San Diego, CA, U.S.A., 1991.

[46] Swinne J, Van Veen J A, Merckx R. 14C pulse- labeling of field- grown spring wheat: an evaluation of its use in rhizosphere carbon budget estimations. Soil Biology and Biochemistry, 1994, 26: 161~170.

[47] Larionova A A, Sapronov D V, Lopes de Gerenyu, V O, Kuznetsova, L G, Kudeyarov, V N. The contribution of root respiration of grasses and trees to the CO2 emission from the soil. Euras. Soil. Science, 2005.

[48] Bloom A J, Caldwell R M. Root excision decreases nutrient adsorption and gas fluxes. Plant and Physiology, 1988, 87: 794~796.

[49] Craine J M, Wedin D A, Chapin F S. Predominance of ecophysiological controls on soil CO2 flux in a Minnesota grassland. Plant and Soil, 1999, 207: 77~86.

[50] Cheng W, Coleman D C, Carroll C R, Hoffman C A. In situ measurement of root respiration and soluble C concentrations in the rhizosphere. Soil Biology and Biochemistry, 1993, 25(9): 1189~1196.

[51] Cheng W, Coleman D C, Carroll C R, Hoffman C A. Investigating short- term carbon flows in the rhizospheres of different plant species, using isotopic trapping. Agronomy Journal 1994, 86: 782~788.

[52] Cheng W, Zhang Q, Coleman D C, Carroll C R, Hoffman C A. Is available carbon limiting microbial respiration in the rhizosphere? Soil Biology & Biochemistry , 1996, 28(10- 11): 1283~1288.

[53] Warembourg F R, Billes G. Estimating carbon transfers in the plant rhizosphere, in Harley J L, Scott Russell, R. (eds.): The Soil- Root interface. Academic Press, London, 1979, pp: 183~196.

[54] Nguyen C, Todorovic C, Robin C, Christophe A, Guckert A. Continuous monitoring of rhizosphere respiration after labeling of plant shoots with 14CO2. Plant and Soil, 1999, 212: 191~201.

[55] Kuzyakov Y, Kretzschmar A, Stahr K. Contribution of Loliun perenne rhizodeposition to carbon turnover of pasture soil. Plant and Soil, 1999, 213: 127~136.

[56] Kuzyakov Y, Cheng W. Photosynthesis controls of rhizosphere respiration and organic matter decomposition. Soil Biology and Biochemistry, 2001, 33: 1915~1925.

[57] Kuzyakov Y, Siniakina S V. Siphon method of separating root- derived organic compounds from root respiration in no- sterile soil. Plant Nutrition and Soil Science, 2001, 164: 511~517.

[58] Jones D L. Organic acids in the rhizosphere- a critical review. Plant and Soil,1998, 205: 25~44.

[59] Jones D L, Darrah P R. Re- sorption of organic compounds by roots of Zea mays L. and its consequences in the rhizosphere. Ⅱ: Experimental and model evidence for simultaneous exudation and re- sorption of soluble C compounds. Plant and Soil, 1993, 153: 47~59.

[60] Jones D L, Darrah P R. Re- sorption of organic compounds by roots of Zea mays L. and its consequences in the rhizosphere. Ⅲ. Characteristics of sugar influx and efflux. Plant and Soil, 1996, 178: 153~160.

[61] Swinnen J. Evaluation of the use of a model rhizodeposition technique to separate root and microbial respiration in soil. Plant and Soil, 1994, 165: 89~101.

[62] Santruckova H, Bird M I, Lloyd J. Microbial processes and carbon- isotope fractionation in tropical and temperate grassland soils. Function Ecology, 2000, 14: 108~114.

[63] Rochette P, Angers D A, Flanagan L B. Maize residue decomposition measurement using soil surface carbon dioxide fluxes and natural abundance of 13C. Soil Science of Society of America Journal, 1999, 63: 1385~1396.

[64] Ryan M C, Aravena R. Combining 13C natural abundance and fumigation–extraction methods to investigate soil microbial biomass turnover. Soil Biology and Biochemistry, 1994, 26: 1583~1585.